| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1 |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Species | |
| Storage | |
| Target | |
| UniProt # |
Immunotherapy Immune Checkpoints
Product Overview
PD-L1 Neutralizing Antibody FAB fragment, recombinant humanized monoclonal, recognizing the extracellular domain of human PD-L1 and blocking the binding of PD-L1 to PD-1. This antibody FAB has been shown to cross-react with mouse PD-L1. No other species have been tested.
| Clonality | Monoclonal |
|---|---|
| Host | Human |
| Antibody Type | IgG1 |
| Isotype | IgG1 |
| Conjugate | Biotin (Avi-Tag) |
| Tag/Label | Heavy Chain: N-terminal Avi-tag |
| Reactivity | Human |
| Purification | Protein A affinity chromatography (HEK293) |
| Molecular Weight | Heavy Chain: 23 kDa, Light Chain: 23 kDa |
| Amino Acids | Heavy Chain: 1-210; Light Chain: 1-214(end) |
| Formulation | 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol. |
| UniProt | Q9NZQ7 |
| Storage | Stable for at least 12 months at -80°C. Protect from light. |
Validated Applications
- Western Blot
Assay conditions: Anti-PD-L1-Biotin (BPS Bioscience #11032) was titrated from 0 to 50 nM with a 2-fold dilution series in presence of 100 nM PD-L1-FLAG (BPS Bioscience #71183) and incubated for 1 hour at RT. Binding was detected with Anti-FLAG-Eu and Streptavidin-APC.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Avoid freeze/thaw cycles.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 71120
- 71214
- 60543
- 72032
This antibody has been validated for: Western Blot. This product carries a Biotin (Avi-Tag) label for direct detection. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at Stable for at least 12 months at -80°C. Protect from light.. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. This product carries a Biotin (Avi-Tag) label for direct detection. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
- Vernon-Wilson, E.F. et al., Eur. J. Immunol. 2000; 30(8): 2130-2137.
- Majeti, R. et al., Cell. 2009; 138(2): 286-299.
