| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Programmed cell death protein 1;Protein PD-1;hPD-1;CD279;PDCD1;PD1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PD1 recombinant protein (Position: P101-L288). Human PD1 shares 59% amino acid (aa) sequence identity with mouse PD1. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PDCD1 (Programmed cell death protein 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PD1/PDCD1 Antibody catalog # RP1039. Tested in IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human PD1 recombinant protein (Position: P101-L288). Human PD1 shares 59% amino acid (aa) sequence identity with mouse PD1. (reported region: P101-L288).
- Molecular weight context: reported MW: nan; calculated MW: nan
- Reactivity: Human
- Applications: IHC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Programmed cell death protein 1; Programmed cell death protein 1. PDCD1 (Programmed cell death 1), also called PD1, encodes a cell surface receptor that is a member of the B7 superfamily involved in immunomodulation. This gene is mapped to 2q37.3. PDCD1 acts as an inhibitory molecule on T cells after interacting with its ligands PDL1 and PDL2. The PDCD1 gene contains 5 exons. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. Using flow cytometric analysis, It has been found that expression of PDCD1 was upregulated on CD16-positive and CD16-negative monocytes, but not on dendritic cells, in viremic HIV-positive patients, but not in highly active antiretroviral therapy (HAART)-treated HIV-positive patients. PDCD1 upregulation in monocytes was induced by microbial Toll-like receptor ligands and inflammatory cytokines. Functional note: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. . Reported localization: Membrane; Single-pass type I membrane protein.
Research relevance and current trends
- Apoptosis: Researchers commonly examine how PDCD1 (Programmed cell death protein 1) relates to this theme using model systems and orthogonal readouts.
- Associated Proteins: Researchers commonly examine how PDCD1 (Programmed cell death protein 1) relates to this theme using model systems and orthogonal readouts.
- Developmental Biology: Researchers commonly examine how PDCD1 (Programmed cell death protein 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of PDCD1 (Programmed cell death protein 1) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
