| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death protein 1; Protein PD-1; mPD-1; CD antigen CD279; Pdcd1; Pd1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse PD1/Pdcd1 recombinant protein (Position: A48-L288). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets programmed cell death protein 1 (gene Pdcd1) in mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 31.8 kDa, and the supplier reports an observed band at about 55 kDa.
PD-1 (PDCD1) is an inhibitory receptor on activated T cells, B cells and other immune cells. When bound by PD-L1 or PD-L2, it recruits the phosphatase SHP-2 to dampen T-cell receptor signaling, helping maintain self-tolerance; blocking PD-1 is the basis of widely used cancer immunotherapies.
| Target | Programmed cell death protein 1 (gene Pdcd1; UniProt Q02242, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 31.8 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse EL-4 cells, mouse spleen tissue, mouse thymus tissue, mouse RAW264.7 cells, mouse Ana-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse thymus tissue, rat thymus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse PD1/Pdcd1 fragment (Ala48–Leu288), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places programmed cell death protein 1 in the cell membrane. Tissue expression noted by UniProt (mouse): Thymus-specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
