| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3',5'-cyclic-AMP phosphodiesterase 4D; DPDE3; PDE43; cAMP-specific phosphodiesterase 4D; PDE4D |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDE4D |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18P16 is a rabbit monoclonal antibody that recognizes 3',5'-cyclic-AMP phosphodiesterase 4D (gene PDE4D) in human samples and is validated for Western blot. The predicted molecular weight is 91.1 kDa, and the supplier reports an observed band at about 91 kDa.
PDE4D is a cAMP-specific phosphodiesterase of the PDE4 family with many isoforms that are anchored to distinct signaling complexes. By degrading cAMP it controls PKA signaling in the heart, airway smooth muscle, immune cells and brain, including β-adrenergic responses, and it is widely studied in cardiovascular, inflammatory and cognitive research.
| Target | 3',5'-cyclic-AMP phosphodiesterase 4D (gene PDE4D; UniProt Q08499, human) |
|---|---|
| Clone | 18P16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 91.1 kDa |
| Observed MW | 91 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PDE4D.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 3',5'-cyclic-AMP phosphodiesterase 4D in the apical cell membrane, cytoplasm and membrane. Tissue expression noted by UniProt (human): Expressed in colonic epithelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Jing et al. (2026) Oncogenic DCTPP1/MYC feedback loop rewires pyrimidine metabolism to drive hepatocellular carcinoma. Functional & Integrative Genomics. 10.1007/s10142-026-01870-8
