| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein disulfide-isomerase A2; Pancreas-specific protein disulfide isomerase; PDIp; PDIA2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PDIP/PDIA2 recombinant protein (Position: Y117-D492). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein disulfide-isomerase A2 (gene PDIA2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 58.2 kDa, and the supplier reports an observed band at about 65 kDa.
Protein disulfide-isomerase A2 (PDIA2, also called PDIp) is a member of the PDI family that is expressed mainly in the pancreas. Besides chaperone activity that limits aggregation of misfolded proteins, it can bind estrogens inside cells and may help regulate their local levels, and it is studied in pancreatic protein folding.
| Target | Protein disulfide-isomerase A2 (gene PDIA2; UniProt Q13087, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.2 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human PCP tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human PDIP/PDIA2 fragment (Tyr117–Asp492), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places protein disulfide-isomerase A2 in the endoplasmic reticulum lumen. Tissue expression noted by UniProt (human): Highly expressed in pancreas (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Faisal Mahmood et al. (2023) Transcriptomics based identification of S100A3 as the key anti-hepatitis B virus factor of 16F16. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2023.114904
