| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3-phosphoinositide-dependent protein kinase 1;hPDK1;2.7.11.1;PDPK1;PDK1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDPK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HOO-16 is a rabbit monoclonal antibody that recognizes 3-phosphoinositide-dependent protein kinase 1 (gene PDPK1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 63.2 kDa, and the supplier reports an observed band at about 63 kDa.
PDPK1 (3-phosphoinositide-dependent protein kinase 1, often called PDK1) is a master kinase of the AGC family. It phosphorylates the activation loop of AKT, S6K, RSK, SGK, PKC isoforms and other kinases, so it is central to insulin and growth factor signaling through PI3K, controlling cell growth, survival and metabolism, and is widely studied in cancer and metabolic research.
| Target | 3-phosphoinositide-dependent protein kinase 1 (gene PDPK1; UniProt O15530, human) |
|---|---|
| Clone | HOO-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.2 kDa |
| Observed MW | 63 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): PC-12 cells.
Immunogen
Synthetic peptide based on the sequence of human PDPK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 3-phosphoinositide-dependent protein kinase 1 in the cytoplasm, nucleus, cell membrane and cell junction. Tissue expression noted by UniProt (human): Appears to be expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Xian et al. (2023) Pyruvate Dehydrogenase Kinase 1 inhibition mediated oxidative phosphorylation enhancement in cartilage promotes osteoarthritis progression. BMC Musculoskeletal Disorders. 10.1186/s12891-023-06585-6
