| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pancreas/duodenum homeobox protein 1;PDX-1;Glucose-sensitive factor;GSF;Insulin promoter factor 1;IPF-1;Insulin upstream factor 1;IUF-1;Islet/duodenum homeobox-1;IDX-1;Somatostatin-transactivating factor 1;STF-1;PDX1;IPF1, STF1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human PDX1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PDX1/Ipf1 Rabbit Monoclonal Antibody is an antibody targeting PDX1. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AEC-16; isotype: Rabbit IgG; reactivity: Human; observed MW: 42 kDa; calculated MW: 30771 MW.
Boster Bio Anti-PDX1/Ipf1 Rabbit Monoclonal Antibody catalog # M00491. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: PDX1 — Pancreas/duodenum homeobox protein 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AEC-16; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 42 kDa; Calculated: 30771 MW
Biological background
Protein function (datasheet): Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
Cellular localization (datasheet): Nucleus. Cytoplasm, cytosol .
Tissue details (datasheet): Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
Research relevance and current trends
- Commonly studied in contexts related to Atherosclerosis,Cancer,Cardiovascular,Developmental Biology,Diabetes,Diabetes-associated,Epigenetics and Nuclear Signaling,Growth Factors,Growth Factors/Hormones,Lineage Markers,Lineage Specification,Metabolism,Organogenesis,Signal Transduction,Stem Cells,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
