| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Peflin; PEF protein with a long N-terminal hydrophobic domain; Penta-EF hand domain-containing protein 1; PEF1; ABP32; UNQ1845; PRO3573 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PEF1 recombinant protein (Position: R170-L284). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PEF1 Antibody Picoband® is an antibody for PEF1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PEF1 (penta-EF-hand domain containing 1); UniProt: Q9UBV8
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 30 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-PEF1 Antibody Picoband® catalog # A09591-2.
Biological background
Biological context: Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR (KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR (KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31.
Expression and localization notes: cellular localization: Endoplasmic reticulum. Cytoplasm. Membrane. Peripheral membrane protein. COPII-coated vesicle membrane. Peripheral membrane protein., tissue context: Pre-B-cells and B-cells but not terminally differentiated plasma cells..
Common research applications
- Western blotting (WB): Compare PEF1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PEF1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify PEF1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Peflin is a protein that in humans is encoded by the PEF1 gene. This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca (2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Endoplasmic reticulum. Cytoplasm. Membrane. Peripheral membrane protein. COPII-coated vesicle membrane. Peripheral membrane protein.
- Tissue details: Pre-B-cells and B-cells but not terminally differentiated plasma cells.
- Research category: Epigenetics and Nuclear Signaling,Mediator Complex,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
