Anti-PEPP2/PLEKHA5 Antibody Picoband®

Overview

Anti-PEPP2/PLEKHA5 Antibody Picoband® is an antibody for PLEKHA5 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: PLEKHA5 (tudor domain containing 3); UniProt: Q9HAU0
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 130 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-PEPP2/PLEKHA5 Antibody Picoband® catalog # A09135-2.

Biological background

Biological context: Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins.

Expression and localization notes: cellular localization: Nucleus. Cytoplasm., tissue context: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas..

Common research applications

• Western blotting (WB): Compare PLEKHA5 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of PLEKHA5 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify PLEKHA5-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Pleckstrin homology domain-containing family A member 5 is a protein that in humans is encoded by the PLEKHA5 gene. This gene, which encodes a transcriptional repressor, is one of two paralogous X-linked homeobox-containing genes and is highly expressed in a variety of cancers. In addition, the encoded protein associates with the cell membrane and with microtubules and is concentrated at the leading edge of migratory cells.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus. Cytoplasm.
• Tissue details: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
• Research category: Adaptive Immunity,B Cells,Immunoglobulins,Immunology

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.