| Field | Specification |
|---|---|
| Target | |
| Alternative names | Period circadian protein homolog 3; hPER3; Cell growth-inhibiting gene 13 protein; Circadian clock protein PERIOD 3; PER3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PER3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24P84 is a rabbit monoclonal antibody that recognizes period circadian protein homolog 3 (gene PER3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 131.9 kDa, and the supplier reports an observed band at about 132 kDa.
PER3 (Period 3) is a member of the Period family of circadian clock proteins. Unlike PER1 and PER2 it is not strictly required for clock function in mice, but it influences circadian timing and sleep regulation, and PER3 gene variants have been associated with diurnal preference and sleep homeostasis in humans.
| Target | Period circadian protein homolog 3 (gene PER3; UniProt P56645, human) |
|---|---|
| Clone | 24P84 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 131.9 kDa |
| Observed MW | 132 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human Caco-2 cells, human HepG2 cells, rat skeletal muscle tissue, rat RH35 cells, mouse skeletal muscle tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PER3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places period circadian protein homolog 3 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhuo Feng et al. (2021) Kaempferol-induced GPER upregulation attenuates atherosclerosis via the PI3K/AKT/Nrf2 pathway. Pharmaceutical Biology. 10.1080/13880209.2021.1961823
