| Field | Specification |
|---|---|
| Target | |
| Alternative names | Perforin-1;P1;Cytolysin;Lymphocyte pore-forming protein;PFP;PRF1;PFP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects perforin-1 (gene PRF1) in human samples and is validated for Western blot. The predicted molecular weight is 61.4 kDa, and the supplier reports an observed band at about 61 kDa.
Perforin-1 (PRF1) is the pore-forming protein stored in the cytotoxic granules of CD8+ T cells and natural killer cells. After calcium-dependent insertion into the target cell membrane it oligomerizes into pores that let granzymes enter and trigger apoptosis, making it a key readout in studies of antiviral and antitumor immunity.
| Target | Perforin-1 (gene PRF1; UniProt P14222, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 61.4 kDa |
| Observed MW | 61 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human HEL cells, human Daudi cells, human HUH7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Perforin fragment (Glu175–Trp555), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 68%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes perforin-1 as secreted and also located in the cytolytic granule, cell membrane and endosome lumen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Heather R. Ferguson Bennit et al. (2021) Natural Killer Cell Phenotype and Functionality Affected by Exposure to Extracellular Survivin and Lymphoma-Derived Exosomes. International Journal of Molecular Sciences. 10.3390/ijms22031255
