| Field | Specification |
|---|---|
| Target | |
| Alternative names | Periostin;PN;Osteoblast-specific factor 2;OSF-2;Postn;Osf2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Periostin, identical to the related rat sequence, and different from the related human sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects periostin (gene Postn) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 93.1 kDa, and the supplier reports an observed band at about 93 kDa.
Periostin (POSTN) is a secreted matricellular protein of the fasciclin family, found in the periosteum, periodontal ligament and other collagen-rich tissues. It binds integrins and matrix proteins to support cell adhesion and collagen crosslinking. Periostin is studied in bone and tooth biology, tissue repair, fibrosis, allergic inflammation and tumor stroma.
| Target | Periostin (gene Postn; UniProt Q62009, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 93.1 kDa |
| Observed MW | 93 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): mouse cardiac muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of mouse periostin. The peptide sequence is conserved in rat. It differs from the human sequence at three residues.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes periostin as secreted and also located in the Golgi apparatus. Tissue expression noted by UniProt (mouse): Preferentially expressed in periosteum and periodontal ligament. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
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