| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PERK recombinant protein (Position: R222-Q334). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes eukaryotic translation initiation factor 2-alpha kinase 3 (gene EIF2AK3) in human samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 125.2 kDa, and the supplier reports an observed band at about 150 kDa.
PERK (EIF2AK3) is an ER membrane kinase that senses unfolded proteins. When activated, it phosphorylates eIF2α to reduce general translation while allowing ATF4 synthesis, making it one of the three main arms of the unfolded protein response.
| Target | Eukaryotic translation initiation factor 2-alpha kinase 3 (gene EIF2AK3; UniProt Q9NZJ5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 125.2 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human 293T cells, human MCF-7 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human pancreas cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human PERK fragment (Arg222–Gln334), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places eukaryotic translation initiation factor 2-alpha kinase 3 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Pei-pei Fang et al. (2020) ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes. Mediators of Inflammation. 10.1155/2020/8183713
Xuefeng Bu et al. (2016) Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress. American Journal of Cancer Research. PMID: 27293989
Yang Zheng et al. (2019) Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways. Brazilian Journal of Medical and Biological Research. 10.1590/1414-431X20199085
Lumei Chi et al. (2019) miR-9-5p attenuates ischemic stroke through targeting ERMP1-mediated endoplasmic reticulum stress. ACTA Histochemica. 10.1016/j.acthis.2019.08.005
Jun Bai et al. (2018) Effects of 5‑hydroxy‑4'‑nitro‑7‑propionyloxy‑genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells. Oncology Letters. 10.3892/ol.2018.7938
Lijun Yang et al. (2025) Neuroprotective effects of dexmedetomidine in spinal cord injury via modulating PERK/ATF4 pathway. Neurological Research. 10.1080/01616412.2025.2527159
Bin Xiao et al. (2013) Endoplasmic reticulum stress in diethylnitrosamine‑induced rat liver cancer. Oncology Letters. 10.3892/ol.2013.1651
