| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxiredoxin-1;1.11.1.15;Natural killer cell-enhancing factor A;NKEF-A;Proliferation-associated gene protein;PAG;Thioredoxin peroxidase 2;Thioredoxin-dependent peroxide reductase 2;PRDX1;PAGA, PAGB, TDPX2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1, different from the related mouse sequence by one amino acid, and identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets peroxiredoxin-1 (gene PRDX1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 24 kDa.
Peroxiredoxin 1 (PRDX1) is an abundant thiol-based peroxidase that reduces hydrogen peroxide and organic hydroperoxides using conserved cysteine residues. Beyond protecting cells from oxidative stress, it helps tune hydrogen peroxide levels that act as signals downstream of growth factors and TNF-α, and it is widely studied in redox biology and cancer research.
| Target | Peroxiredoxin-1 (gene PRDX1; UniProt Q06830, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.1 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Raji cells, human Jurkat cells, human MDA-MB-453 cells, human HeLa cells, human HepG2 cells, human HEK293 cells, human placenta tissue, human A549 cells, rat kidney tissue, rat testis tissue, rat liver tissue, rat PC-12 cells, mouse kidney tissue, mouse testis tissue, mouse liver tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells, U2OS cells.
Samples with confirmed staining (ICC/IF): SMMC-7721 cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Synthetic peptide from the middle region of human peroxiredoxin 1. The peptide sequence is conserved in rat. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places peroxiredoxin-1 in the cytoplasm and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Guo-Qiang et al. (2020) Prdx1 Reduces Intracerebral Hemorrhage-Induced Brain Injury via Targeting Inflammation- and Apoptosis-Related mRNA Stability. Frontiers in Neuroscience. 10.3389/fnins.2020.00181
Yujuan You et al. (2024) Remifentanil represses oxidative stress to relieve hepatic ischemia/reperfusion injury via regulating BACH1/PRDX1 axis. Clinics and Research in Hepatology and Gastroenterology. 10.1016/j.clinre.2024.102422
You Xiangrong et al. (2014) Cloning and molecular characterization of phospholipase D (PLD) delta gene from longan (Dimocarpus longan Lour.). Molecular Biology Reports. 10.1007/s11033-014-3306-3
