Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband®

Overview

This antibody is intended for detection of PRDX3 (Thioredoxin-dependent peroxide reductase, mitochondrial) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband® catalog # PB9349. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E.coli-derived human Peroxiredoxin 3 recombinant protein (Position: T110-Q256). Human Peroxiredoxin 3 shares 93% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 3. (reported region: T110-Q256).
• Molecular weight context: reported MW: 25 kDa; calculated MW: 27693 MW
• Reactivity: Human,Mouse,Rat
• Applications: Flow Cytometry, IF, IHC, ICC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Thioredoxin-dependent peroxide reductase, mitochondrial; Thioredoxin-dependent peroxide reductase, mitochondrial. PRDX3 (peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function. Functional note: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. . Reported localization: Mitochondrion. Expression/tissue context: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Research relevance and current trends

• Invasion/Microenvironment: Researchers commonly examine how PRDX3 (Thioredoxin-dependent peroxide reductase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
• Metabolism: Researchers commonly examine how PRDX3 (Thioredoxin-dependent peroxide reductase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
• Metabolism Processes: Researchers commonly examine how PRDX3 (Thioredoxin-dependent peroxide reductase, mitochondrial) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative PRDX3 (Thioredoxin-dependent peroxide reductase, mitochondrial) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of PRDX3 (Thioredoxin-dependent peroxide reductase, mitochondrial) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
• Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Family / similarity context: Belongs to the AhpC/TSA family.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.