| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxiredoxin-5, mitochondrial;1.11.1.15;Alu corepressor 1;Antioxidant enzyme B166;AOEB166;Liver tissue 2D-page spot 71B;PLP;Peroxiredoxin V;Prx-V;Peroxisomal antioxidant enzyme;TPx type VI;Thioredoxin peroxidase PMP20;Thioredoxin reductase;PRDX5;ACR1;SBBI10; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes peroxiredoxin-5, mitochondrial (gene PRDX5) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 22 kDa.
Peroxiredoxin 5 (PRDX5) is an atypical 2-Cys peroxiredoxin found in mitochondria, peroxisomes and the cytosol. It reduces hydrogen peroxide, alkyl hydroperoxides and peroxynitrite, helping protect cells against oxidative and nitrosative stress, and it is used as a marker in studies of redox balance and organelle protection.
| Target | Peroxiredoxin-5, mitochondrial (gene PRDX5; UniProt P30044, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.1 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): A549 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lung cancer tissue, rat intestine tissue, mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with confirmed staining (ICC/IF): SMMC cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human Peroxiredoxin 5 fragment (Glu66–Asp198), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
