| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Peroxiredoxin-6;1.11.1.15;1-Cys peroxiredoxin;1-Cys PRX;24 kDa protein;Acidic calcium-independent phospholipase A2;aiPLA2;3.1.1.-;Antioxidant protein 2;Liver 2D page spot 40;Non-selenium glutathione peroxidase;NSGPx;1.11.1.9;Red blood cells page spot 12;PRDX6;AOP2, KIAA0106; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PRDX6 (Peroxiredoxin-6) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Peroxiredoxin 6/PRDX6 Antibody Picoband® catalog # PB9350. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively. (reported region: E15-P224).
- Molecular weight context: reported MW: 25 kDa; calculated MW: 25035 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Peroxiredoxin-6; Peroxiredoxin-6. PRDX6 is also known as PRX, p29 or AOP2. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H (2)O (2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Functional note: Involved in redox regulation of the cell. Can reduce H (2)O (2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Reported localization: Cytoplasm . Lysosome . Cytoplasmic vesicle . Also found in lung secretory organelles. . Expression/tissue context: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
Research relevance and current trends
- Atherosclerosis: Researchers commonly examine how PRDX6 (Peroxiredoxin-6) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how PRDX6 (Peroxiredoxin-6) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how PRDX6 (Peroxiredoxin-6) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PRDX6 (Peroxiredoxin-6) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of PRDX6 (Peroxiredoxin-6) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Family / similarity context: Belongs to the AhpC/TSA family. Rehydrin subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
