| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Integrin-linked protein kinase;2.7.11.1;59 kDa serine/threonine-protein kinase;ILK-1;ILK-2;p59ILK;ILK;ILK1, ILK2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human PGC1 beta |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PPARGC1B antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEOI-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-PGC1 beta PPARGC1B Monoclonal Antibody catalog # M02933. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PPARGC1B (Integrin-linked protein kinase).
- Antibody format: Monoclonal; clone AEOI-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PPARGC1B (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. Reported cellular localization context: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere. Tissue expression notes (as provided): Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cardiovascular,Co-Activators/Co-Repressors,Diabetes,Diabetes-associated,Energy Metabolism,Energy Transfer Pathways,Epigenetics and Nuclear Signaling,Fatty Acid Oxidation,Fatty Acids,Heart Disease,Lipid and Lipoprotein Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Nuclear Receptors,Nuclear Signaling Pathways,Obesity,Pathways and Processes,Redox Metabolism,Signal Transduction,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PPARGC1B antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PPARGC1B expression by Western blot in cell or tissue lysates, Enrich PPARGC1B by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 113 kDa; calculated MW: 51419 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 113 kDa
- Cellular localization (provided): Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere.
- Tissue details (provided): Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
