| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxisome proliferator-activated receptor gamma coactivator 1-beta; PGC-1-beta; PPAR-gamma coactivator 1-beta; PPARGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PPARGC1B; PERC; PGC1; PGC1B; PPARGC1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PGC1 beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEOI-16 is a rabbit monoclonal antibody that recognizes peroxisome proliferator-activated receptor gamma coactivator 1-beta (gene PPARGC1B) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 113.2 kDa, and the supplier reports an observed band at about 113 kDa.
PGC-1β (PPARGC1B) is a transcriptional coactivator closely related to PGC-1α. It enhances the activity of estrogen receptor α, NRF1 and other factors and supports basal mitochondrial biogenesis and oxidative metabolism. It is studied in lipid metabolism, mitochondrial function and energy balance.
| Target | Peroxisome proliferator-activated receptor gamma coactivator 1-beta (gene PPARGC1B; UniProt Q86YN6, human) |
|---|---|
| Clone | AEOI-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 113.2 kDa |
| Observed MW | 113 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HeLa cells, mouse spleen, rat spleen, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PGC1 beta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places peroxisome proliferator-activated receptor gamma coactivator 1-beta in the nucleus. Tissue expression noted by UniProt (human): Ubiquitous with higher expression in heart, brain and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
