| Field | Specification |
|---|---|
| Target | |
| Alternative names | 6-phosphogluconate dehydrogenase, decarboxylating; PGD; PGDH |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from PGD |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26P73 is a rabbit monoclonal antibody that recognizes 6-phosphogluconate dehydrogenase, decarboxylating (gene PGD) in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 53.1 kDa, and the supplier reports an observed band at about 43 kDa.
6-phosphogluconate dehydrogenase (PGD) catalyzes the third step of the oxidative pentose phosphate pathway, oxidatively decarboxylating 6-phosphogluconate to yield ribulose 5-phosphate, CO2 and NADPH. It thus supplies NADPH for antioxidant defense and biosynthesis as well as pentose sugars for nucleotides, and it is studied in redox and cancer metabolism.
| Target | 6-phosphogluconate dehydrogenase, decarboxylating (gene PGD; UniProt P52209, human) |
|---|---|
| Clone | 26P73 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.1 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): 293 cells, mouse kidney, rat kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of PGD.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 6-phosphogluconate dehydrogenase, decarboxylating in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
