| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphoglycerate kinase 1;2.7.2.3;Cell migration-inducing gene 10 protein;Primer recognition protein 2;PRP 2;PGK1;PGKA;MIG10, OK/SW-cl.110; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PGK1, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phosphoglycerate kinase 1 (gene PGK1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 44.6 kDa, and the supplier reports an observed band at about 43 kDa.
Phosphoglycerate kinase 1 (PGK1) catalyzes the first ATP-producing step of glycolysis, transferring a phosphate from 1,3-bisphosphoglycerate to ADP to give 3-phosphoglycerate. It is ubiquitously expressed and X-linked, and in mitochondria it can also act as a protein kinase that phosphorylates PDK1 to favor glycolysis. PGK1 is studied in cancer metabolism.
| Target | Phosphoglycerate kinase 1 (gene PGK1; UniProt P00558, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 44.6 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human 293T cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U87 cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Synthetic peptide from the C-terminal region of human PGK1. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphoglycerate kinase 1 in the cytoplasm and mitochondrion matrix. Tissue expression noted by UniProt (human): Mainly expressed in spermatogonia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jing Zhou et al. (2023) Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice. Life Sciences. 10.1016/j.lfs.2023.121491
Jingjing Liu et al. (2021) Terazosin Stimulates Pgk1 to Remedy Gastrointestinal Disorders. International Journal of Molecular Sciences. 10.3390/ijms23010416
Jingjing Liu et al. (2025) Repurposing Lamivudine: A novel therapeutic strategy for ischemic stroke targeting the glycolytic enzyme Pgk1. Biochemical Pharmacology. 10.1016/j.bcp.2025.117529
