| Field | Specification |
|---|---|
| Target | |
| Alternative names | Egl nine homolog 1; Hypoxia-inducible factor prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2; Prolyl hydroxylase domain-containing protein 2; PHD2; SM-20; EGLN1; C1orf12 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PHD2 / prolyl hydroxylase. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACEF-5 is a rabbit monoclonal antibody that recognizes egl nine homolog 1 (gene EGLN1) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 46.0 kDa, and the supplier reports an observed band at about 50 kDa.
EGLN1 (also called PHD2) is the main cellular oxygen sensor for hypoxia-inducible factor. In normal oxygen it hydroxylates prolines in HIF-α, marking it for VHL-mediated degradation, while in hypoxia HIF-α is stabilized. It is studied in hypoxia signaling, erythropoiesis and cancer.
| Target | Egl nine homolog 1 (gene EGLN1; UniProt Q9GZT9, human) |
|---|---|
| Clone | ACEF-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.0 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human PHD2 / prolyl hydroxylase.
Reactivity Notes
The supplier lists reactivity with human. UniProt places egl nine homolog 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
