| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;2.7.11.1 ;Acetyl-CoA carboxylase kinase;ACACA kinase;2.7.11.27 ;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;2.7.11.31 ;Tau-protein kinase PRKAA1;2.7.11.26;PRKAA1;AMPK1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human AMPK alpha 1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-AMPK alpha 1 (S496) PRKAA1 Rabbit Monoclonal Antibody is an antibody targeting PRKAA1. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HDH-16; isotype: Rabbit IgG; reactivity: Human; observed MW: 80 kDa; calculated MW: 64009 MW.
Boster Bio Anti-Phospho-AMPK alpha 1 (S496) PRKAA1 Rabbit Monoclonal Antibody catalog # P00994-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: PRKAA1 — 5'-AMP-activated protein kinase catalytic subunit alpha-1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HDH-16; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 80 kDa; Calculated: 64009 MW
- Phospho site(s): S496
Biological background
Protein function (datasheet): Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by inly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. .
Cellular localization (datasheet): Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters.
Tissue details (datasheet): Ubiquitously expressed, with more abundant expression in the brain.
Research relevance and current trends
- Commonly studied in contexts related to Alzheimer's Disease,Cancer,Cancer Metabolism,Cardiovascular,Energy Transfer Pathways,Fatty Acid Oxidation,Fatty Acids,Integration Of Energy,Integration Of Energy Metabolism,Lipid and Lipoprotein Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism Processes,Neurodegenerative Disease,Neurology Process,Neuroscience,Pathways and Processes,Protein Phosphorylation,Redox Metabolism,Response To Hypoxia,Ser/Thr Kinases,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
