| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cyclic AMP-dependent transcription factor ATF-2;cAMP-dependent transcription factor ATF-2;2.3.1.48 ;Activating transcription factor 2;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding protein 2;HB16;Histone acetyltransferase ATF2;cAMP response element-binding protein CRE-BP1;ATF2;CREB2, CREBP1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-ATF2 (T71) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-ATF2 (T71) Rabbit Monoclonal Antibody is an antibody targeting ATF2. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: FOO-1; isotype: Rabbit IgG; reactivity: Human; observed MW: 80 kDa; calculated MW: 54537 MW.
Boster Bio Anti-Phospho-ATF2 (T71) Rabbit Monoclonal Antibody catalog # P00916-3. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: ATF2 — Cyclic AMP-dependent transcription factor ATF-2
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: FOO-1; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 80 kDa; Calculated: 54537 MW
- Phospho site(s): T71
Biological background
Protein function (datasheet): Transcriptional activator which regulates the transcription of various genes, including those involved in anti- apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. .
Cellular localization (datasheet): Nucleus. Cytoplasm. Mitochondrion outer membrane. Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co- localizes with the MRN complex in the IR-induced foci (IRIF).
Tissue details (datasheet): Ubiquitously expressed, with more abundant expression in the brain.
Research relevance and current trends
- Commonly studied in contexts related to Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Immunology,Innate Immunity,TLR Signaling,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
