| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor AP-1;Activator protein 1;AP1;Proto-oncogene c-Jun;V-jun avian sarcoma virus 17 oncogene homolog;p39;JUN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Jun |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HDG-10 is a rabbit monoclonal antibody that recognizes transcription factor Jun phosphorylated at Thr91 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 35.7 kDa, and the supplier reports an observed band at about 45 kDa.
c-Jun (JUN) is a bZIP transcription factor and core component of AP-1. It forms dimers with Fos family proteins and other partners to bind TRE (TGA[GC]TCA) sites and control genes involved in proliferation, differentiation, stress responses and apoptosis. Its activity is strongly increased by JNK phosphorylation, and it is a widely used readout in studies of cell signaling and cancer.
| Target | Transcription factor Jun (gene JUN; UniProt P05412, human) — phosphorylated at Thr91 |
|---|---|
| Clone | HDG-10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.7 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human c-Jun.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor Jun in the nucleus. Tissue expression noted by UniProt (human): Expressed in the developing and adult prostate and prostate cancer cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hua Liu et al. (2024) Using broadly targeted plant metabolomics technology combined with network pharmacology to explore the mechanism of action of the Yishen Gushu formula in the treatment of postmenopausal osteoporosis in vivo. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118469
