Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody

SKU:BHA21009305
Research Validated
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Overview
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Anti-Phospho-ERK1 (T202) + ERK2 (T185) antibody (Rabbit monoclonal, clone 18M06, IgG). Validated for Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation. Reactivity: Human, Mouse, Rat. Observed band in Western blot: about 42, 44 kDa.
Target MAPK3
clone number 18M06
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB, IHC, ICC, IF, IP
Options selector
Catalog no. Size Conjugation
P00104-2 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC,ICC,IF,IP
      Application details: WB 1:500-1:1000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:40
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MAPK3
Alternative names Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;2.7.11.24;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p44-ERK1;MAPK3;ERK1, PRKM3;
Gene ID 5595
UniProt # P27361/P28482
Host Rabbit
Clone 18M06
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185)
Purification Affinity-chromatography
Cellular localization Cytoplasm. Nucleus. Autophosphorylation at Thr-207 promotes nuclear localization.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) P00104-2
Main SKU BHA21009305
Rabbit Monoclonal · Phospho-specific

Product Overview

Clone 18M06 is a rabbit monoclonal antibody that recognizes MAPK3 phosphorylated at Thr202 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation.

Target MAPK3 (UniProt P27361/P28482) — phosphorylated at Thr202
Clone 18M06
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Observed MW 42, 44 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:1000
Immunohistochemistry 1:50–1:200
Immunocytochemistry / Immunofluorescence 1:50–1:200
Immunoprecipitation 1:40

Immunogen

Synthetic phosphopeptide around Thr202 of human ERK1 + ERK2.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:1000; Immunohistochemistry 1:50–1:200; Immunocytochemistry / Immunofluorescence 1:50–1:200; Immunoprecipitation 1:40. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: observed bands at about 42 and 44 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic phosphopeptide around Thr202 of human ERK1 + ERK2.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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C. Yu et al. (2005) Inhibition of the PI3K pathway sensitizes fludarabine-induced apoptosis in human leukemic cells through an inactivation of MAPK-dependent pathway. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2005.03.182

Huanyue Cui et al. (2021) Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/6103158

Wen Lili et al. (2016) Role of the Endoplasmic Reticulum Pathway in the Medial Prefrontal Cortex in Post-Traumatic Stress Disorder Model Rats. Journal of Molecular Neuroscience. 10.1007/s12031-016-0755-2

Yuqin Hao et al. (2013) The inhibition of resveratrol to human skin squamous cell carcinoma A431 xenografts in nude mice. Fitoterapia. 10.1016/j.fitote.2013.02.005

Wang Liqing et al. (2021) The negative charge of the 343 site is essential for maintaining physiological functions of CXCR4. BMC Molecular and Cell Biology. 10.1186/s12860-021-00347-9

Hai-Rong Ma et al. (2014) Icariin and icaritin stimulate the proliferation of SKBr3 cells through the GPER1-mediated modulation of the EGFR-MAPK signaling pathway. International Journal of Molecular Medicine. 10.3892/ijmm.2014.1722

Lin Luo et al. (2017) Buyang Huanwu Decoction Ameliorates Poststroke Depression via Promoting Neurotrophic Pathway Mediated Neuroprotection and Neurogenesis. Evidence-based Complementary and Alternative Medicine. 10.1155/2017/4072658

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