| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Forkhead box protein O3;AF6q21 protein;Forkhead in rhabdomyosarcoma-like 1;FOXO3;FKHRL1, FOXO3A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human FoxO3a |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody is an antibody targeting FOXO3. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ECG-6; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 80-100 kDa; calculated MW: 70 kDa.
Boster Bio Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody catalog # P00252. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: FOXO3 — Forkhead box protein O3
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ECG-6; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 80-100 kDa; Calculated: 70 kDa
- Phospho site(s): S253
Biological background
Protein function (datasheet): Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. .
Cellular localization (datasheet): Cytoplasm, cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
Tissue details (datasheet): Ubiquitous. .
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Associated Proteins,Cancer,Cell Biology,Co-Activators/Co-Repressors,Domain Families,Epigenetics and Nuclear Signaling,Forkhead Box,Hypoxia,Intracellular,Metabolism,Metabolism Processes,Nuclear Receptors,Nuclear Signaling Pathways,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes,Transcription,Transcription Factors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
