| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone H1.4;Histone H1b;Histone H1s-4;HIST1H1E;H1F4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-Histone H1.4 (T17) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-Histone H1.4 (T17) HIST1H1E Rabbit Monoclonal Antibody is an antibody targeting HIST1H1E. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: DGH-8; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 36 kDa; calculated MW: 21865 MW.
Boster Bio Anti-Phospho-Histone H1.4 (T17) HIST1H1E Rabbit Monoclonal Antibody catalog # P06652. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: HIST1H1E — Histone H1.4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: DGH-8; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 36 kDa; Calculated: 21865 MW
- Phospho site(s): T17
Biological background
Protein function (datasheet): Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). .
Cellular localization (datasheet): Nucleus. Chromosome. Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.
Tissue details (datasheet): Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Research relevance and current trends
- Commonly studied in contexts related to DNA/RNA,DNA Damage & Repair,Epigenetics and Nuclear Signaling,Histones,Unmodified.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
