| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Serine/threonine-protein kinase/endoribonuclease IRE1;Endoplasmic reticulum-to-nucleus signaling 1;Inositol-requiring protein 1;hIRE1p;Ire1-alpha;IRE1a;Serine/threonine-protein kinase;2.7.11.1;Endoribonuclease;3.1.26.-;ERN1 ;IRE1 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-IRE1 (S724) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody is an antibody targeting ERN1. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: EGH-5; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 185 kDa; calculated MW: 109735 MW.
Boster Bio Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody catalog # P00683. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ERN1 — Serine/threonine-protein kinase/endoribonuclease IRE1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: EGH-5; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 185 kDa; Calculated: 109735 MW
- Phospho site(s): S724
Biological background
Protein function (datasheet): Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. .
Cellular localization (datasheet): Endoplasmic reticulum membrane ; Single-pass type I membrane protein .
Tissue details (datasheet): Ubiquitously expressed. High levels observed in pancreatic tissue. .
Research relevance and current trends
- Commonly studied in contexts related to Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
