| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2; JAK2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-JAK2 (Y1007 + Y1008) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IFE-10 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase JAK2 phosphorylated at Tyr1007 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 130.7 kDa, and the supplier reports an observed band at about 130 kDa.
JAK2 is a cytoplasmic tyrosine kinase of the Janus kinase family that associates with receptors for erythropoietin, thrombopoietin, growth hormone, prolactin, leptin and interferons. Ligand binding activates JAK2, which phosphorylates the receptor and recruits STAT proteins such as STAT5 that then move to the nucleus to switch on target genes. The JAK2 V617F mutation is well known in myeloproliferative neoplasms, so JAK2 is widely studied in hematopoiesis and blood cancers.
| Target | Tyrosine-protein kinase JAK2 (gene JAK2; UniProt O60674, human) — phosphorylated at Tyr1007 |
|---|---|
| Clone | IFE-10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 130.7 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:30 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human cervix cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): Jurkat cells.
Immunogen
Synthetic phosphopeptide around Tyr1007 of human JAK2 +.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein kinase JAK2 in the endomembrane system, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed throughout most tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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