| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;2.7.11.24;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;MNK1;Microtubule-associated protein 2 kinase;p44-ERK1;Mapk3;Erk1, Prkm3; |
| Cellular Localization | |
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| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH. |
| Isotype | |
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Overview
Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) is an antibody targeting MAPK1; MAPK3. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: MAPK-YT; isotype: Mouse IgG1; reactivity: Human,Mouse,Rat,Yeast; observed MW: 42 kDa; calculated MW: 43081 MW.
Boster Bio Anti-Phospho-MAP Kinase, Activated (Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) catalog # MA1055. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Yeast.
Key elements and design rationale
- Target: MAPK1; MAPK3 — Mitogen-activated protein kinase 3
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: MAPK-YT; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rat,Yeast
- Molecular weight guidance: Observed: 42 kDa; Calculated: 43081 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Scientific background (datasheet): In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
Cellular localization (datasheet): Cytoplasm . Nucleus. Autophosphorylation at Thr-207 promotes nuclear localization. PEA15-binding res the biological outcome of MAPK3 kinase- signaling by sequestering MAPK3 into the cytoplasm. .
Tissue details (datasheet): Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung.
Sequence similarities (datasheet): Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Research relevance and current trends
- Commonly studied in contexts related to Alzheimer's Disease,Cytoplasmic,MAPK Pathway,Neurodegenerative Disease,Neurology Process,Neuroscience,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
