| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Red fluorescent protein eqFP611;GFP-like chromoprotein; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-MSK1 (S376) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-RPS6KA5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone FCF-18; isotype Rabbit IgG; reactivity: Human,Rat. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal Antibody catalog # MP04922. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: RPS6KA5 (Red fluorescent protein eqFP611).
- Antibody format: Monoclonal; clone FCF-18; isotype Rabbit IgG.
- Host: Rabbit.
- PTM context: Phospho site information provided: S376.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
RPS6KA5 is a commonly studied target in molecular and cellular biology. Functional context (as provided): Pigment protein. . Reported cellular localization context: Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. . Tissue expression notes (as provided): Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Research relevance and current trends
- Research context keywords from the source record include: Epigenetics and Nuclear Signaling,Metabolism,Pathways and Processes,Signal Transduction,Transcription,Cell Biology,Protein Phosphorylation,Ser/Thr Kinases,Tyrosine Kinases,Receptor Tyrosine Kinases,Oxidative Stress,Redox Metabolism.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
- When phosphorylation is relevant, researchers frequently track stimulus-driven changes and compare phospho-state to total protein abundance.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate RPS6KA5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect RPS6KA5 expression by Western blot in cell or tissue lysates, Detect RPS6KA5 in FFPE tissue sections by immunohistochemistry, Localize RPS6KA5 by immunofluorescence/immunocytochemistry in cultured cells, Enrich RPS6KA5 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 15 kDa; calculated MW: 26053 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 15 kDa
- Cellular localization (provided): Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .
- Tissue details (provided): Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
