| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinoblastoma-associated protein;p105-Rb;pRb;Rb;pp110;RB1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Rb (S807) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DGG-18 is a rabbit monoclonal antibody that recognizes retinoblastoma-associated protein (gene RB1) phosphorylated at Ser807 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 106.2 kDa, and the supplier reports an observed band at about 106 kDa.
The retinoblastoma protein (pRb, RB1) is a classic tumor suppressor that controls entry into S phase. In its underphosphorylated form it binds E2F transcription factors and recruits chromatin-modifying enzymes to silence cell cycle genes; phosphorylation by cyclin–CDK complexes releases E2F and allows DNA replication to begin. Loss of RB1 causes retinoblastoma, and pRb status is a standard readout in cell cycle and cancer research.
| Target | Retinoblastoma-associated protein (gene RB1; UniProt P06400, human) — phosphorylated at Ser807 |
|---|---|
| Clone | DGG-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 106.2 kDa |
| Observed MW | 106 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human K562 cells, human Jurkat cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human spleen tissue, mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic phosphopeptide around Ser807 of human rb.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places retinoblastoma-associated protein in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in the retina. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ruan Yunjun et al. (2014) Retarding the senescence of human vascular endothelial cells induced by hydrogen peroxide: effects of 17beta-estradiol (E2) mediated mitochondria protection. Biogerontology. 10.1007/s10522-014-9507-2
Xingsheng Hu et al. (2024) The expression and role of SUZ12 in lung adenocarcinoma. Cancer Medicine. 10.1002/cam4.70190
Xu Tang et al. (2016) Molecular Mechanism of Enhanced Anticancer Effect of Nanoparticle Formulated LY2835219 via p16-CDK4/6-pRb Pathway in Colorectal Carcinoma Cell Line. Journal of Nanomaterials. 10.1155/2016/2095878
