| Field | Specification |
|---|---|
| Target | |
| Alternative names | BDNF/NT-3 growth factors receptor;2.7.10.1;GP145-TrkB;Trk-B;Neurotrophic tyrosine kinase receptor type 2;TrkB tyrosine kinase;Tropomyosin-related kinase B;NTRK2;TRKB; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-TrkB (Y817) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EGA-14 is a rabbit monoclonal antibody that recognizes BDNF/NT-3 growth factors receptor (gene NTRK2) phosphorylated at Tyr817 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 92.0 kDa, and the supplier reports an observed band at about 130 kDa.
TrkB (NTRK2) is a receptor tyrosine kinase activated mainly by BDNF and neurotrophin-4. Its signaling supports neuron survival, differentiation and synapse formation and is central to activity-dependent synaptic plasticity, so TrkB is widely studied in learning and memory, depression, neurodegeneration and obesity.
| Target | BDNF/NT-3 growth factors receptor (gene NTRK2; UniProt Q16620, human) — phosphorylated at Tyr817 |
|---|---|
| Clone | EGA-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 92.0 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): mouse brain cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic phosphopeptide around Tyr817 of human trkB.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places BDNF/NT-3 growth factors receptor in the cell membrane, endosome membrane, early endosome membrane and cell projection. Tissue expression noted by UniProt (human): Isoform TrkB is expressed in the central and peripheral nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Esteban-Ortega Gema María et al. (2025) Retrograde transport of neurotrophin receptor TrkB-FL induced by excitotoxicity regulates Golgi stability and is a target for stroke neuroprotection. Cell Death & Disease. 10.1038/s41419-025-07990-6
Fan Fang-Cheng et al. (2023) Carboxypeptidase E conditional knockout mice exhibit learning and memory deficits and neurodegeneration. Translational Psychiatry. 10.1038/s41398-023-02429-y
Qiong Wang et al. (2019) Doxorubicin and adjudin co-loaded pH-sensitive nanoparticles for the treatment of drug-resistant cancer. Acta Biomaterialia. 10.1016/j.actbio.2019.05.061
Mei Jiang et al. (2023) Yueliang Yin Ameliorates Endometrial Receptivity in Mice with Embryo Implantation Failure by Reducing Pyroptosis and Activating BDNF/TrkB Pathway. Molecular Nutrition & Food Research. 10.1002/mnfr.202300339
Bin Liu et al. (2018) Inhibition of protein tyrosine phosphatase 1B protects against sevoflurane-induced neurotoxicity mediated by ER stress in developing brain. Brain Research Bulletin. 10.1016/j.brainresbull.2018.12.006
Fei Fei et al. (2024) Comparison of CUMS at different pregnancy stages, maternal separation, and their effects on offspring in postpartum depression mouse models. Heliyon. 10.1016/j.heliyon.2024.e35363
Wen-Ling Lin et al. (2025) Exercise-Induced Weight Loss Modulates Brain Iron and Omega-3 Fatty Acid Metabolism in Middle-Aged Obese Rats. Nutrition. 10.1016/j.nut.2025.112872
