| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone H2A.2;HTA2;H2A2;YBL003C;YBL0103; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from Phosphotyrosine |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-Tyrosine Rabbit Monoclonal Antibody is an antibody targeting Tyrosine. Common applications include WB, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AAOI-20; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 140 kDa, 130 kDa, 110 kDa; calculated MW: 13989 MW.
Boster Bio Anti-Phospho-Tyrosine Rabbit Monoclonal Antibody catalog # PT0005. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: Tyrosine — Histone H2A.2
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AAOI-20; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 140 kDa, 130 kDa, 110 kDa; Calculated: 13989 MW
Biological background
Protein function (datasheet): Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. .
Cellular localization (datasheet): Nucleus. Chromosome.
Tissue details (datasheet): Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Research relevance and current trends
- Commonly studied in contexts related to Epigenetics and Nuclear Signaling,Histones.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
