| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytosolic phospholipase A2;cPLA2;Phospholipase A2 group IVA;Phospholipase A2;3.1.1.4;Phosphatidylcholine 2-acylhydrolase;Lysophospholipase;3.1.1.5;PLA2G4A;CPLA2, PLA2G4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cytosolic phospholipase A2 (gene PLA2G4A) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 85.2 kDa, and the supplier reports an observed band at about 85 kDa.
Cytosolic phospholipase A2 α (cPLA2α) is the key enzyme that releases arachidonic acid from membrane phospholipids when cells are stimulated. Activated by Ca2+ binding and phosphorylation, it supplies the precursor for prostaglandins and leukotrienes and is therefore central to studies of inflammation, pain and reproduction.
| Target | Cytosolic phospholipase A2 (gene PLA2G4A; UniProt P47712, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 85.2 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|
Samples with a confirmed band (WB): HeLa cells, NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 40 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human PLA2G4A. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places cytosolic phospholipase A2 in the cytoplasm, Golgi apparatus membrane and nucleus envelope. Tissue expression noted by UniProt (human): Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hao Wu et al. (2024) Chaihu Guizhi Ganjiang Decoction attenuates nonalcoholic steatohepatitis by enhancing intestinal barrier integrity and ameliorating PPARα mediated lipotoxicity. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117841
