| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3-kinase regulatory subunit gamma;PI3-kinase regulatory subunit gamma;PI3K regulatory subunit gamma;PtdIns-3-kinase regulatory subunit gamma;Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma;PI3-kinase subunit p55-gamma;PtdIns-3-kinase regulatory subunit p55-gamma;p55PIK;PIK3R3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PI 3 Kinase p55 gamma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DHC-16 is a rabbit monoclonal antibody that recognizes phosphatidylinositol 3-kinase regulatory subunit gamma (gene PIK3R3) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 54.4 kDa, and the supplier reports an observed band at about 50 kDa.
PIK3R3 encodes p55γ, a regulatory subunit of class IA PI3K. Through its SH2 domains it binds phosphorylated tyrosine kinases and insulin receptor substrate 1, helping couple receptor activation to PI3K signaling.
| Target | Phosphatidylinositol 3-kinase regulatory subunit gamma (gene PIK3R3; UniProt Q92569, human) |
|---|---|
| Clone | DHC-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.4 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human SH-SY5Y cells, human Raji cells, human Daudi cells, rat brain tissue, mouse brain tissueIHC: human pituitary tumor tissue, human lung adenocarcinoma tissue, human pituitary tumor tissue, human astrocytoma tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): C6 cells.
Immunogen
Synthetic peptide based on the sequence of human PI 3 Kinase p55 gamma.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Highest levels in brain and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Xiaomeng et al. (2025) Covariation of scleral remodeling and PI3K/Akt signaling pathway in experimental myopia. Scientific Reports. 10.1038/s41598-025-97643-7
Xudong Cheng et al. (2024) The Therapeutic Mechanisms of Shenyan Oral Liquid I Against Chronic Kidney Disease Based on Network Pharmacology and Experimental Validation. Combinatorial Chemistry & High Throughput Screening. 10.2174/0113862073260994231031070916
