| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Phosphatidylinositol 3-kinase regulatory subunit gamma;PI3-kinase regulatory subunit gamma;PI3K regulatory subunit gamma;PtdIns-3-kinase regulatory subunit gamma;Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma;PI3-kinase subunit p55-gamma;PtdIns-3-kinase regulatory subunit p55-gamma;p55PIK;PIK3R3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human PI 3 Kinase p55 gamma |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PIK3R3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone DHC-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody catalog # M06707. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PIK3R3 (Phosphatidylinositol 3-kinase regulatory subunit gamma).
- Antibody format: Monoclonal; clone DHC-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PIK3R3 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Immunology,Innate Immunity,Lipid Signaling,Signal Transduction,Signaling Pathway,TLR Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate PIK3R3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PIK3R3 expression by Western blot in cell or tissue lysates, Detect PIK3R3 in FFPE tissue sections by immunohistochemistry, Localize PIK3R3 by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 130 kDa, 270 kDa; calculated MW: 54448 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 130 kDa, 270 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
