| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3-kinase regulatory subunit beta;PI3-kinase regulatory subunit beta;PI3K regulatory subunit beta;PtdIns-3-kinase regulatory subunit beta;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta;PI3-kinase subunit p85-beta;PtdIns-3-kinase regulatory subunit p85-beta;PIK3R2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets phosphatidylinositol 3-kinase regulatory subunit beta (gene PIK3R2) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 81.5 kDa, and the supplier reports an observed band at about 82 kDa.
PIK3R2 encodes p85β, a regulatory subunit of class IA PI3K that binds and restrains the p110 catalytic subunit and links it to activated tyrosine kinase receptors. It helps control PIP3 production and AKT signaling, and activating PIK3R2 mutations are known causes of megalencephaly-polymicrogyria syndromes.
| Target | Phosphatidylinositol 3-kinase regulatory subunit beta (gene PIK3R2; UniProt O00459, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 81.5 kDa |
| Observed MW | 82 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human A549 cells, human Jurkat cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Synthetic peptide from the middle region of human PIK3R2. It differs from the mouse sequence at two residues and from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guangxiao Ni et al. (2024) MicroRNA-199a-5p attenuates blood-brain barrier disruption following ischemic stroke by regulating PI3K/Akt signaling pathway. PLoS One. 10.1371/journal.pone.0306793
Tianyang Xi et al. (2017) MicroRNA-126-3p attenuates blood-brain barrier disruption, cerebral edema and neuronal injury following intracerebral hemorrhage by regulating PIK3R2 and Akt. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.10.064
