| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase pim-1;2.7.11.1;PIM1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PIM1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ECB-16 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase pim-1 (gene PIM1) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 35.7 kDa, and the supplier reports an observed band at about 34 kDa.
PIM1 is a constitutively active serine/threonine kinase induced by cytokine and JAK/STAT signaling. It promotes cell survival and proliferation by phosphorylating BAD and other apoptosis regulators, enhancing MYC activity and influencing cell cycle proteins, and it is overexpressed in many blood cancers and solid tumors, making it a drug target.
| Target | Serine/threonine-protein kinase pim-1 (gene PIM1; UniProt P11309, human) |
|---|---|
| Clone | ECB-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.7 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human k562 cells, human HEL cells, human RT4 cells, human HepG2 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human prostate carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PIM1.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Expressed primarily in cells of the hematopoietic and germline lineages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaomeng Ji et al. (2025) PIM-1 exacerbates sepsis-associated encephalopathy via promoting microglia NLRP3 inflammasome activation. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2025.09.055
Yuan-Bo Wu et al. (2016) PIM1 polymorphism and PIM1 expression as predisposing factors of esophageal squamous cell carcinoma in the Asian population. OncoTargets and Therapy. 10.2147/OTT.S103392
