| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pituitary homeobox 3; Homeobox protein PITX3; Paired-like homeodomain transcription factor 3; PITX3; PTX3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PITX3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26P12 is a rabbit monoclonal antibody that recognizes pituitary homeobox 3 (gene PITX3) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 31.8 kDa, and the supplier reports an observed band at about 36 kDa.
PITX3 is a paired-like homeodomain transcription factor needed for the development and survival of midbrain dopaminergic neurons and for lens formation. Together with NURR1 it activates dopaminergic genes such as tyrosine hydroxylase and the dopamine transporter, making it an important marker in Parkinson's disease and stem cell differentiation research.
| Target | Pituitary homeobox 3 (gene PITX3; UniProt O75364, human) |
|---|---|
| Clone | 26P12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.8 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): rat skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PITX3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places pituitary homeobox 3 in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in developing eye lens. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chunjin Li et al. (2016) BDNF-induced expansion of cumulus-oocyte complexes in pigs was mediated by microRNA-205. Theriogenology. 10.1016/j.theriogenology.2016.01.004
