| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAC-alpha serine/threonine-protein kinase;2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;RAC-PK-alpha;Akt1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone PKB-175 is a mouse monoclonal antibody that recognizes RAC-alpha serine/threonine-protein kinase (gene Akt1) in chicken, human, mouse and rat samples and is validated for Western blot.
AKT1 (protein kinase Bα) is one of three AKT serine/threonine kinases activated downstream of PI3K by growth factors and insulin. By phosphorylating many substrates, it promotes cell survival, growth, proliferation, glucose uptake and angiogenesis, and it is one of the most studied nodes in cancer and metabolic signaling.
| Target | RAC-alpha serine/threonine-protein kinase (gene Akt1; UniProt P47196, rat) |
|---|---|
| Clone | PKB-175 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Chicken, Human, Mouse, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat, Chicken) |
|---|
Immunogen
Synthetic peptide based on the sequence of corresponding to amino acids 461–477 of human PKB alpha/Akt1, conjugated to KLH.
Reactivity Notes
The supplier lists reactivity with chicken, human, mouse and rat. UniProt places RAC-alpha serine/threonine-protein kinase in the cytoplasm, nucleus, cell membrane and mitochondrion intermembrane space. Tissue expression noted by UniProt (rat): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chaohui Wang et al. (2026) Rhodiosin promotes cerebral angiogenesis in mice with ischemic stroke via PI3K/Akt pathway activation. Phytomedicine. 10.1016/j.phymed.2026.158247
Shi Yongjing et al. (2024) The E3 ubiquitin ligase FBXO38 maintains the antitumor function of natural killer cells by sustaining IL-15R signaling. Cancer Immunology Research. 10.1158/2326-6066.CIR-23-1061
Lu Kong et al. (2021) Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells. Environmental Pollution. 10.1016/j.envpol.2021.116556
Jing Zhou et al. (2023) Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice. Life Sciences. 10.1016/j.lfs.2023.121491
Xie Feng et al. (2021) Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology. Frontiers in Pharmacology. 10.3389/fphar.2021.609702
Li Yang et al. (2018) Galangin Alleviates Liver Ischemia-Reperfusion Injury in a Rat Model by Mediating the PI3K/AKT Pathway. Cellular Physiology and Biochemistry. 10.1159/000495553
Ning Zhang et al. (2026) Safflower washing medicine alleviating acute soft tissue injury via PI3K/Akt pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2026.121239
Zhiwang Wang et al. (2025) Therapeutic mechanism of Jinshui Liujunzi Decoction in treating COPD: targeting the PI3K/Akt signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120070
Thomson Patrick Joseph et al. (2021) Duloxetine ameliorates valproic acid-induced hyperactivity, anxiety-like behavior, and social interaction deficits in zebrafish. Autism Research. 10.1002/aur.2620
Maria Chiara Magnifico et al. (2018) Nonylphenol and Octylphenol Differently Affect Cell Redox Balance by Modulating the Nitric Oxide Signaling. Oxidative Medicine and Cellular Longevity. 10.1155/2018/1684827
