| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein kinase C delta type;2.7.11.13;Tyrosine-protein kinase PRKCD;2.7.10.2;nPKC-delta;Protein kinase C delta type regulatory subunit;Protein kinase C delta type catalytic subunit;Sphingosine-dependent protein kinase-1;SDK1;PRKCD; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PKC delta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IHD-16 is a rabbit monoclonal antibody that recognizes protein kinase C delta type (gene PRKCD) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 77.5 kDa, and the supplier reports an observed band at about 78 kDa.
PKCδ (PRKCD) is a novel protein kinase C isoform activated by diacylglycerol independently of calcium. It has context-dependent roles in apoptosis and survival, becoming pro-apoptotic after DNA damage, and it also regulates NADPH oxidase activity and B-cell tolerance, so it is studied in cell death, immunity and cancer.
| Target | Protein kinase C delta type (gene PRKCD; UniProt Q05655, human) |
|---|---|
| Clone | IHD-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.5 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PKC delta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein kinase C delta type in the cytoplasm, nucleus, cell membrane and mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cui Huilin et al. (2021) The expression of diacylglycerol kinase isoforms α and ζ correlates with the progression of experimental autoimmune encephalomyelitis in rats. Histochemistry and Cell Biology. 10.1007/s00418-021-02011-x
Xuan Wang et al. (2024) Curcumin Alleviates Osteoarthritis Through the p38MAPK Pathway: Network Pharmacological Prediction and Experimental Confirmation. Journal of Inflammation Research. 10.2147/JIR.S459867
Zhang Xian-Ming et al. (2004) Apoptosis of murine melanoma B16-BL6 cells induced by quercetin targeting mitochondria, inhibiting expression of PKC-α and translocating PKC-δ. Cancer Chemotherapy and Pharmacology. 10.1007/s00280-004-0863-5
