| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein kinase C eta type;2.7.11.13;PKC-L;nPKC-eta;PRKCH;PKCL, PRKCL; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PKC eta recombinant protein (Position: R30-D389). Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein kinase C eta type (gene PRKCH) in human samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 77.8 kDa, and the supplier reports an observed band at about 78 kDa.
PKCη (PRKCH) is a calcium-independent, diacylglycerol-activated novel protein kinase C enriched in epithelial tissues. In keratinocytes it promotes growth arrest and differentiation, and it also helps maintain epithelial tight junctions by phosphorylating occludin, so it is studied in skin biology, barrier function and T-cell signaling.
| Target | Protein kinase C eta type (gene PRKCH; UniProt P24723, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 77.8 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): A431 cells, A549 cells, HeLa cells, SKOV cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): A431 cells, K562 cells, MCF-7 cells.
Immunogen
Recombinant human PKC eta fragment (Arg30–Asp389), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.7% and 96.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places protein kinase C eta type in the cytoplasm. Tissue expression noted by UniProt (human): Most abundant in lung, less in heart and skin. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Taiping Gan et al. (2016) 5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors. Drug Design Development and Therapy. 10.2147/DDDT.S93570
