| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pyruvate kinase PKLR;2.7.1.40;Pyruvate kinase 1;Pyruvate kinase isozymes L/R;R-type/L-type pyruvate kinase;Red cell/liver pyruvate kinase;PKLR;PK1, PKL; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR, different from the related mouse sequence by one amino acid, and identical to the rat sequence. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PKLR (Pyruvate kinase PKLR) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PKLR Antibody Picoband® catalog # PB9499. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR, different from the related mouse sequence by one amino acid, and identical to the rat sequence.
- Molecular weight context: reported MW: 62 kDa; calculated MW: 61830 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Pyruvate kinase PKLR; Pyruvate kinase PKLR. Pyruvate kinase isozymes R/L is an enzyme that in humans is encoded by the PKLR gene. It is mapped to 1q21. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. Functional note: Plays a key role in glycolysis. . Reported localization: Nucleus. Nucleus, nucleolus. Chromosome, telomere. Chromosome, centromere, kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE. Expression/tissue context: Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
Research relevance and current trends
- Cancer: Researchers commonly examine how PKLR (Pyruvate kinase PKLR) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how PKLR (Pyruvate kinase PKLR) relates to this theme using model systems and orthogonal readouts.
- Carbohydrate Metabolism: Researchers commonly examine how PKLR (Pyruvate kinase PKLR) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PKLR (Pyruvate kinase PKLR) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of PKLR (Pyruvate kinase PKLR) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the pyruvate kinase family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
