| Field | Specification |
|---|---|
| Target | |
| Alternative names | 85/88 kDa calcium-independent phospholipase A2; CaI-PLA2; Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; Patatin-like phospholipase domain-containing protein 9; PNPLA9; PLA2G6; PLPLA9 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PLA2G6 recombinant protein (Position: W715-L802). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 85/88 kDa calcium-independent phospholipase A2 (gene PLA2G6) in human, mouse and rat samples and is validated for ELISA, ICC/IF and Western blot. The predicted molecular weight is 89.9 kDa, and the supplier reports an observed band at about 84 kDa.
PLA2G6 (iPLA2β) is a Ca2+-independent phospholipase A2 that remodels membrane phospholipids and supports mitochondrial membrane integrity. Mutations in PLA2G6 cause PLA2G6-associated neurodegeneration, including infantile neuroaxonal dystrophy, and certain forms of early-onset parkinsonism.
| Target | 85/88 kDa calcium-independent phospholipase A2 (gene PLA2G6; UniProt O60733, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 89.9 kDa |
| Observed MW | 84 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells, human Jurkat cells, human 293T cells, human K562 cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Recombinant human PLA2G6 fragment (Trp715–Leu802), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 85/88 kDa calcium-independent phospholipase A2 in the cytoplasm, cell membrane, mitochondrion and cell projection. Tissue expression noted by UniProt (human): Four different transcripts were found to be expressed in a distinct tissue distribution. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qing Rao et al. (2026) Retrorsine Induces Hepatotoxicity through ATF3-Mediated Ferroptosis in Mouse Primary Hepatocytes and CYP3A4-HepG2 Cells. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.6c01532
