| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phospholipase A-2-activating protein; PLA2P; PLAP; PLAA |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PLAP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25P22 is a rabbit monoclonal antibody that recognizes phospholipase A-2-activating protein (gene PLAA) in human samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 87.2 kDa, and the supplier reports an observed band at about 75 kDa.
PLAA (phospholipase A2-activating protein) is a cofactor of the p97/VCP segregase that helps sort ubiquitinated membrane proteins into the endolysosomal pathway. It supports lysosomal clearance of damaged membranes and synaptic function, and loss-of-function mutations cause a severe neurodevelopmental disorder.
| Target | Phospholipase A-2-activating protein (gene PLAA; UniProt Q9Y263, human) |
|---|---|
| Clone | 25P22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 87.2 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PLAP.
Reactivity Notes
The supplier lists reactivity with human. UniProt places phospholipase A-2-activating protein in the nucleus, cytoplasm and synapse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Yang et al. (2023) Placenta-derived exosomes as carriers of non-coding RNAs in maternal circulation of patients with preeclampsia. Biotechnology & Genetic Engineering Reviews. 10.1080/02648725.2023.2202511
