| Field | Specification |
|---|---|
| Target | |
| Alternative names | Plasminogen; Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A, short form; Plasmin light chain B; PLG |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Plasminogen, which shares 72.7% amino acid (aa) sequence identity with both mouse and rat Plasminogen. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes plasminogen (gene PLG) in human, mouse and rat samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 90.6 kDa, and the supplier reports an observed band at about 100 kDa.
Plasminogen is the liver-derived zymogen of plasmin, the main enzyme that dissolves fibrin clots. Converted to plasmin by tPA or uPA, it also breaks down extracellular matrix proteins and activates growth factors and metalloproteinases, so it is central to research on fibrinolysis, wound healing and tissue remodeling.
| Target | Plasminogen (gene PLG; UniProt P00747, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 90.6 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HL-60 cells, human HEK293 cells, human Caco-2 cells, human Sw620 cells, human Jurkat cells, human K562 cells, rat liver tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Synthetic peptide from the N-terminal region of human plasminogen. Sequence identity with the mouse and rat orthologs: 72.7%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places plasminogen in the [Plasmin]: Secreted. Tissue expression noted by UniProt (human): Present in plasma and many other extracellular fluids. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dejun Yang et al. (2021) Increased plasmin-mediated proteolysis of L1CAM in a mouse model of idiopathic normal pressure hydrocephalus. Proceedings of the National Academy of Sciences of the United States of America. 10.1073/pnas.2010528118
Shuizhong Han et al. (2024) Mycoplasma synoviae LP78 is a fibronectin/plasminogen binding protein, putative adhesion, and potential diagnostic antigen. Frontiers in Microbiology. 10.3389/fmicb.2023.1335658
Wang Jia et al. (2022) A multifunctional enolase mediates cytoadhesion and interaction with host plasminogen and fibronectin in Mycoplasma hyorhinis. Veterinary Research. 10.1186/s13567-022-01041-0
Zhu Weifeng et al. (2017) Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in recruitment of host fibronectin and plasminogen. Veterinary Research. 10.1186/s13567-017-0421-x
Zhu Weifeng et al. (2017) Erysipelothrix rhusiopathiae recruits host plasminogen via the major protective antigen SpaA. Fems Microbiology Letters. 10.1093/femsle/fnx036
