| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Plasminogen; Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A, short form; Plasmin light chain B; PLG |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Plasminogen, which shares 72.7% amino acid (aa) sequence identity with both mouse and rat Plasminogen. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PLG (Breast cancer metastasis-suppressor 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Plasminogen/PLG Antibody Picoband® catalog # A03674-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human Plasminogen, which shares 72.7% amino acid (aa) sequence identity with both mouse and rat Plasminogen.
- Molecular weight context: reported MW: 100 kDa; calculated MW: 28461 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Breast cancer metastasis-suppressor 1; plasminogen. Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU) and tissue plasminogen activator (PLAT). The main function of plasmin is to dissolve fibrin (see, e.g., FGA) clots. Plasmin, like trypsin, belongs to the family of serine proteinases. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Functional note: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Reported localization: Secreted. Expression/tissue context: Present in plasma and many other extracellular fluids. It is synthesized in the liver.
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how PLG (Breast cancer metastasis-suppressor 1) relates to this theme using model systems and orthogonal readouts.
- Platelets: Researchers commonly examine how PLG (Breast cancer metastasis-suppressor 1) relates to this theme using model systems and orthogonal readouts.
- Protease Inhibitors: Researchers commonly examine how PLG (Breast cancer metastasis-suppressor 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PLG (Breast cancer metastasis-suppressor 1) levels across conditions; band patterns may reflect isoforms and processing.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
