| Field | Specification |
|---|---|
| Target | |
| Alternative names | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3; PLCB3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PLCB3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24P35 is a rabbit monoclonal antibody that recognizes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (gene PLCB3) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 138.8 kDa, and the supplier reports an observed band at about 150 kDa.
PLCB3 (PLCβ3) is a widely expressed phospholipase C activated by Gαq/11 and Gβγ downstream of G protein-coupled receptors. By generating IP3 and diacylglycerol, it triggers Ca2+ release and PKC activation, and it is studied in neutrophil signaling, sensory neurons and GPCR pharmacology.
| Target | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (gene PLCB3; UniProt Q01970, human) |
|---|---|
| Clone | 24P35 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 138.8 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PLCB3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 in the cytoplasm, membrane and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
