| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;3.1.4.11;PLC-148;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-II;PLC-II;Phospholipase C-gamma-1;PLC-gamma-1;PLCG1;PLC1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human PLCG1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody is an antibody targeting PLCG1. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: FAA-16; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 149 kDa; calculated MW: 148532 MW.
Boster Bio Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody catalog # M00677. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PLCG1 — 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: FAA-16; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 149 kDa; Calculated: 148532 MW
Biological background
Protein function (datasheet): Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. .
Cellular localization (datasheet): Cell projection, lamellipodium. Cell projection, ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Tissue details (datasheet): Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. .
Research relevance and current trends
- Commonly studied in contexts related to Lipid Metabolism,Lipid Signaling,Metabolism,Signal Transduction,Signaling Pathway.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
