| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2; PLD2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human PLD2 recombinant protein (Position: A37-E924). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PLD2 Picoband® Antibody is an antibody for PLD2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PLD2 (phospholipase D2); UniProt: O14939; NCBI Gene: 5338
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 106 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-PLD2 Picoband® Antibody catalog # A02586.
Biological background
Biological context: May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Expression and localization notes: cellular localization: Membrane. Peripheral membrane protein., tissue context: Widely expressed..
Common research applications
- Western blotting (WB): Compare PLD2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PLD2 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify PLD2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Phospholipase D2 is an enzyme that in humans is encoded by the PLD2 gene. It is mapped to 17p13.2. The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane. Peripheral membrane protein.
- Tissue details: Widely expressed.
- Research category: Adaptive Immunity,Autoimmune,B Cells,Cell Type Markers,Cytokines,Immune System Diseases,Immunology,Innate Immunity,T Cells,TNF Superfamily
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
