| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc finger and BTB domain-containing protein 16;Promyelocytic leukemia zinc finger protein;Zinc finger protein 145;Zinc finger protein PLZF;ZBTB16;PLZF, ZNF145; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Plzf recombinant protein (Position: M1-E165). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects zinc finger and BTB domain-containing protein 16 (gene ZBTB16) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 74.3 kDa, and the supplier reports an observed band at about 74 kDa.
ZBTB16, also known as PLZF (promyelocytic leukemia zinc finger), is a BTB/POZ-domain zinc finger transcriptional repressor. It is needed for limb and skeletal patterning, spermatogonial stem cell maintenance and the development of innate-like T cells such as NKT cells, and its fusion with RARA occurs in a rare form of acute promyelocytic leukemia.
| Target | Zinc finger and BTB domain-containing protein 16 (gene ZBTB16; UniProt Q05516, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 74.3 kDa |
| Observed MW | 74 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human Ramos cells, human HeLa cells, rat testis tissue, rat PC-12 cells, mouse testis tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Plzf fragment (Met1–Glu165), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places zinc finger and BTB domain-containing protein 16 in the nucleus. Tissue expression noted by UniProt (human): Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Lingling et al. (2022) Epigenetic Regulation of TET1-SP1 During Spermatogonia Self-Renewal and Proliferation. Frontiers in Physiology. 10.3389/fphys.2022.843825
Lei Shi et al. (2020) Effects of selenium on the proliferation and apoptosis of sheep spermatogonial stem cells in vitro. Animal Reproduction Science. 10.1016/j.anireprosci.2020.106330
