| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Zinc finger and BTB domain-containing protein 16;Promyelocytic leukemia zinc finger protein;Zinc finger protein 145;Zinc finger protein PLZF;ZBTB16;PLZF, ZNF145; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Plzf recombinant protein (Position: M1-E165). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ZBTB16 (Zinc finger and BTB domain-containing protein 16) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Plzf/ZBTB16 Antibody Picoband® catalog # PB9969. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Plzf recombinant protein (Position: M1-E165). (reported region: M1-E165).
- Molecular weight context: reported MW: 74 kDa; calculated MW: 74274 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Zinc finger and BTB domain-containing protein 16; Zinc finger and BTB domain-containing protein 16. Zinc finger and BTB domain-containing protein 16 is a protein that in humans is encoded by the ZBTB16 gene. This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. Functional note: Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. . Reported localization: Nucleus. Expression/tissue context: Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
Research relevance and current trends
- Adaptive Immunity: Researchers commonly examine how ZBTB16 (Zinc finger and BTB domain-containing protein 16) relates to this theme using model systems and orthogonal readouts.
- Cell Type Markers: Researchers commonly examine how ZBTB16 (Zinc finger and BTB domain-containing protein 16) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how ZBTB16 (Zinc finger and BTB domain-containing protein 16) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ZBTB16 (Zinc finger and BTB domain-containing protein 16) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
